We then removed an EcoRI site in it using SOE PCR.
Step1 Primers: ccgGAATTCagcacccaagtgtgca (Fragment1-F)
               Gcaggatacgtccccggattact (Fragment1-R)
               Ggggacgtatcctgcacaatggc (Fragment2-F)
               Ggtctagagggaccacgaccagcagaa (Fragment2-R)
Step2 Primers:  Fragment1-F and Fragment2-R
